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Tsui KH et al. · Reproduction & fertility ·
The work used a human granulosa cell line, HGL5, as a model of aging, plus eggs taken from young and aged mice and kept alive outside the body. MOTS-c was given to the cells and to the eggs in culture. In the cells, the researchers measured mitochondrial mass, fluorescence tied to ATP, reactive oxygen species, membrane potential, and respiration readouts including ATP-linked and maximal respiration, spare respiratory capacity and proton leak. They also measured the levels of enzymes in glycolysis and pyruvate metabolism and of a set of proteins including NRF2, RHOT1, ZO-1, E-cadherin, phosphorylated FAK and AMH. In the eggs they counted first polar body extrusion and measured fluorescence for oxidative stress and for mitochondria. Public transcriptomic datasets from human folliculogenesis and from nonhuman primate ovarian aging were reanalysed alongside.
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