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TB-500 in the published literature

This page describes research published by other people. It is not a claim about the product sold on this site, and listing a study here is not an endorsement of it.

Entries are selected by search query and date, before their results are known to the selector. Where a compound has null or negative findings in the published record, they are listed here alongside the rest.

Where a paper specifies quantities or a route, those details stay in the paper. Follow the link to read them in their original context.

Last reviewed: 2026-08-13

What the published record looks like

Thymosin beta-4 is an endogenous protein studied far beyond this catalogue: a PubMed search on 13 August 2026 for TB-500 or thymosin beta-4 returns 621 records, most of them about sepsis, corneal biology and cardiac tissue. Narrowing that search to tendon, dermal and skeletal contexts leaves 36 records, and four of those are described below: two rodent studies, one bench study of cells on a scaffold, and one analytical study that combined rats with cell assays. They come from four separate research groups on three continents. No trial in people appears anywhere in this set, and the oldest of the four records is from 2010.

Search recorded: ("TB-500"[tiab] OR "thymosin beta-4"[tiab]) AND (tendon[tiab] OR dermal[tiab] OR skeletal[tiab]) · 2026-08-13

Studies listed on this page

Effects of BPC-157 and TB-500 on Achilles tendon healing in rats: A histopathological and biomechanical study

Published: 2026-07-23

Source: Joint diseases and related surgery
Study design: Animal study
Studied in: Male Sprague-Dawley rats, 12 weeks old
Sample size: 32 rats, randomised into four groups of eight
Reported outcome: Difference measured

The same rat study that appears on the BPC-157 page, read here for its TB-500 arm. Of 32 rats whose Achilles tendon was transected and surgically reattached, the eight given TB-500 produced tendons that took significantly more force before failing than the untreated controls, and scored significantly lower on both of the structural grading scales the authors used. Combining TB-500 with BPC-157 produced no further difference over either one alone, which the authors suggest may mean the two act on the same downstream pathway. The paper states the quantities, the route and the schedule it used; all of them stay in its methods section.

Read the record on PubMedPMID 42542926

Search recorded: ("TB-500"[tiab] OR "thymosin beta-4"[tiab]) AND (tendon[tiab] OR dermal[tiab] OR skeletal[tiab]) ·

Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro

Published: 2024-03-01

Source: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Study design: In vitro
Studied in: Rats for the metabolite work; cultured fibroblasts and serum for the assays
Sample size: Parent compound plus its identified breakdown fragments, compared against control
Reported outcome: No measurable difference

An analytical chemistry group built a method that measures TB-500 and its breakdown fragments at the same time, then applied it to samples from rats, to serum, and to enzyme systems on the bench. Two fragments dominated: one appeared early and one persisted for as long as 72 hours. The same fragments were then run through a scratch assay on cultured fibroblasts. Neither TB-500 itself nor most of its fragments produced a significant difference against control in that assay — only the fragment Ac-LKKTE did. The authors' own conclusion is that activity reported for TB-500 elsewhere in the literature may belong to that metabolite rather than to the compound as supplied.

Read the record on PubMedPMID 38382158

Search recorded: ("TB-500"[tiab] OR "thymosin beta-4"[tiab]) AND (tendon[tiab] OR dermal[tiab] OR skeletal[tiab]) · 2026-08-13

Evaluation of skeletal and cardiac muscle function after chronic administration of thymosin beta-4 in the dystrophin deficient mouse

Published: 2010-01-29

Source: PLoS One
Study design: Animal study
Studied in: Female mdx mice and wild-type C57BL10/ScSnJ controls, 8-10 weeks old at start
Sample size: Four groups: treated and untreated mdx, treated and untreated wild type
Reported outcome: Mixed across measures

Female mdx mice — the standard rodent model of Duchenne muscular dystrophy — were given thymosin beta-4 or nothing over six months, and exercised throughout to provoke the phenotype. The paper states the quantities and the schedule it used. The count of regenerating fibres in skeletal tissue rose significantly in the treated mdx group, and staining located the peptide specifically in those regenerating fibres. Grip strength did not change significantly. Cardiac systolic function, measured as percent shortening fraction, did not change significantly. Fibrosis in skeletal and cardiac tissue did not change significantly either. On three of the four things this study measured, the difference between treated and untreated mdx mice failed to reach significance.

Read the record on PubMedPMID 20126456

Search recorded: ("TB-500"[tiab] OR "thymosin beta-4"[tiab]) AND (tendon[tiab] OR dermal[tiab] OR skeletal[tiab]) · 2026-08-13

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TB-500 in the published literature

What the published record looks like

Studies listed on this page

Effects of BPC-157 and TB-500 on Achilles tendon healing in rats: A histopathological and biomechanical study

Product Information

Certificate of Analysis

All products are sold for Research Use Only.

TB-500 in the published literature

What the published record looks like

Studies listed on this page

Effects of BPC-157 and TB-500 on Achilles tendon healing in rats: A histopathological and biomechanical study

Product Information

Certificate of Analysis

Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro

Electrospun thymosin Beta-4 loaded PLGA/PLA nanofiber/ microfiber hybrid yarns for tendon tissue engineering application

Evaluation of skeletal and cardiac muscle function after chronic administration of thymosin beta-4 in the dystrophin deficient mouse

TB-500 in the published literature

What the published record looks like

Studies listed on this page

Effects of BPC-157 and TB-500 on Achilles tendon healing in rats: A histopathological and biomechanical study

Related Resources

Product Information

Certificate of Analysis

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TB-500 in the published literature

What the published record looks like

Studies listed on this page

Effects of BPC-157 and TB-500 on Achilles tendon healing in rats: A histopathological and biomechanical study

Related Resources

Product Information

Certificate of Analysis

Simultaneous quantification of TB-500 and its metabolites in in-vitro experiments and rats by UHPLC-Q-Exactive orbitrap MS/MS and their screening by wound healing activities in-vitro

Electrospun thymosin Beta-4 loaded PLGA/PLA nanofiber/ microfiber hybrid yarns for tendon tissue engineering application

Evaluation of skeletal and cardiac muscle function after chronic administration of thymosin beta-4 in the dystrophin deficient mouse